Growth factor loaded in situ photocrosslinkable poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/gelatin methacryloyl hybrid patch for diabetic wound healing

Management of chronic diabetic ulcers remains as a major challenge in healthcare which requires extensive multidisciplinary approaches to ensure wound protection, management excess exudates and promoting healing. Developing healing patches that can act protective barrier support is highly needed manage ulcers. In order boost the potential patch material, bioactive agents such growth factors be used. Porous membranes made nanofibers generated using electrospinning have for application coverage matrices. However, electrospun produced from several biodegradable polymers are hydrophobic cannot by wounds. Gelatin-methacryloyl (GelMA) hydrogels absorb provide an optimal biological environment wound. Epidermal factor (EGF) promotes cell migration, angiogenesis overall Poly(3-hydroxybutyrate- co -3-hydroxyvalerate) (PHBV) microbial, thermal mechanical properties patch. Herein, we developed polymeric based on combination mechanically stable PHBV, GelMA hydrogel EGF vitro vivo studies were carried out evaluate effect proliferation, Our results showed loaded promote migration proliferation multiple types cells (keratinocytes, fibroblasts endothelial cells) enhance angiogenesis. situ development subsequent study rats rapid compared control Interestingly, 100 ng per cm 2 was enough favourable cellular response, Overall indicate PHBV-GelMA hybrid could promising approach • photocrosslinking exudate capacity while PHBV strength. facilitate